Evaluation of EryC as Molecular Marker for Differential Identification of Brucella abortus from Vaccine Strain S 19

Background: To prevent the losses caused by brucellosis, development of diagnostic tools for differentiating vaccinated cattle from infected one is priority in eradication disease. The present study was undertaken differential identification Brucella abortus vaccine strain S19 EryC PCR. Methods: In this study, 1145 clinical samples unvaccinated and buffaloes (200 blood samples, 710 sera, 190 vaginal swabs, 20 abomasal contents foetus, 25 foetal tissues) 146 animals were collected dairy farms around Mumbai Pune region. These processed isolation organisms further characterized Result: PCR yielded amplicon 1257 bp B. 544, melitensis Rev1, all field isolates, 67 23 tissue 80 (40%) 21 animals. Only two out showed 555 bp. developed during found useful wild type strains also effective when tried on reference samples.